Reporter

Part:BBa_K1144005:Design

Designed by: Silvia J Cañas   Group: iGEM13_Colombia_Uniandes   (2013-09-18)


GAL4 activation reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 84
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

pGAL1 was extracted form Saccharomyces cerevisiae (BBa_K1144001) and fused with the KOZAC sequence using Phusion PCR. Parts BBa_J06504 and BBa_K530019 were obtained from the 2013 Distribution kit and fused using Phusion PCR

References